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immunofluorescence labeling  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc immunofluorescence labeling
    Pain in TRAP‐Cre; Slit3 −/− mice is reduced. a) Experimental design for TRAP‐Cre transgenic mice injected with AAV9‐Slit3‐RNAi. b) <t>Immunofluorescence</t> of SLIT3 (red) in condyles. The white dashed line represents the boundary between subchondral bone and cartilage. Scale bars:10 µm. c) Quantitative analysis in panel (b). n = 6. d) Representative results of OFT and EPM. e,f) Quantitative analysis of Von Frey experiment (e), OFT and EPM (f). n = 6. Statistical analyses were performed using Student's t ‐test in (c), and one‐way ANOVA with Holm–Šidák multiple comparison tests in (e,f). ** p < 0.01. *** p < 0.001. **** p < 0.0001. ns : no significance.
    Immunofluorescence Labeling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunofluorescence+labeling/pmc12866685-288-25-30?v=Cell+Signaling+Technology+Inc
    Average 86 stars, based on 1 article reviews
    immunofluorescence labeling - by Bioz Stars, 2026-07
    86/100 stars

    Images

    1) Product Images from "Osteoclast‐Derived SLIT3 Mediates Osteoarthritis Pain and Degenerative Changes"

    Article Title: Osteoclast‐Derived SLIT3 Mediates Osteoarthritis Pain and Degenerative Changes

    Journal: Advanced Science

    doi: 10.1002/advs.202517545

    Pain in TRAP‐Cre; Slit3 −/− mice is reduced. a) Experimental design for TRAP‐Cre transgenic mice injected with AAV9‐Slit3‐RNAi. b) Immunofluorescence of SLIT3 (red) in condyles. The white dashed line represents the boundary between subchondral bone and cartilage. Scale bars:10 µm. c) Quantitative analysis in panel (b). n = 6. d) Representative results of OFT and EPM. e,f) Quantitative analysis of Von Frey experiment (e), OFT and EPM (f). n = 6. Statistical analyses were performed using Student's t ‐test in (c), and one‐way ANOVA with Holm–Šidák multiple comparison tests in (e,f). ** p < 0.01. *** p < 0.001. **** p < 0.0001. ns : no significance.
    Figure Legend Snippet: Pain in TRAP‐Cre; Slit3 −/− mice is reduced. a) Experimental design for TRAP‐Cre transgenic mice injected with AAV9‐Slit3‐RNAi. b) Immunofluorescence of SLIT3 (red) in condyles. The white dashed line represents the boundary between subchondral bone and cartilage. Scale bars:10 µm. c) Quantitative analysis in panel (b). n = 6. d) Representative results of OFT and EPM. e,f) Quantitative analysis of Von Frey experiment (e), OFT and EPM (f). n = 6. Statistical analyses were performed using Student's t ‐test in (c), and one‐way ANOVA with Holm–Šidák multiple comparison tests in (e,f). ** p < 0.01. *** p < 0.001. **** p < 0.0001. ns : no significance.

    Techniques Used: Transgenic Assay, Injection, Immunofluorescence, Comparison

    Immunofluorescence imaging and analysis of sensory nerve distribution in the condyle of Slit3 ‐knockdown mice. a) Representative images of immunofluorescence staining for PGP9.5 (red), CGRP (green) and DAPI (blue) along the TMJ osteochondral junction. Scale bars: 10 µm. b) Quantitative analysis of the density of PGP9.5 + nerve fibers and CGRP + nerve fibers in the TMJ osteochondral junction. n = 6. c) Schematic diagram of anterograde tracing. d) Representative images of the condylar sensory nerve. EGFP (rAAV‐CAG‐EGFP‐WPRE‐hGH pA, green), DAPI (blue). Scale bars: 10 µm. e) Quantitative analysis of the density of sensory nerve fibers. n = 6. The white dashed line represents the boundary between bone and cartilage. Statistical analyses were performed using one‐way ANOVA with Holm–Šidák multiple comparison tests. **** p < 0.0001. ns : no significance.
    Figure Legend Snippet: Immunofluorescence imaging and analysis of sensory nerve distribution in the condyle of Slit3 ‐knockdown mice. a) Representative images of immunofluorescence staining for PGP9.5 (red), CGRP (green) and DAPI (blue) along the TMJ osteochondral junction. Scale bars: 10 µm. b) Quantitative analysis of the density of PGP9.5 + nerve fibers and CGRP + nerve fibers in the TMJ osteochondral junction. n = 6. c) Schematic diagram of anterograde tracing. d) Representative images of the condylar sensory nerve. EGFP (rAAV‐CAG‐EGFP‐WPRE‐hGH pA, green), DAPI (blue). Scale bars: 10 µm. e) Quantitative analysis of the density of sensory nerve fibers. n = 6. The white dashed line represents the boundary between bone and cartilage. Statistical analyses were performed using one‐way ANOVA with Holm–Šidák multiple comparison tests. **** p < 0.0001. ns : no significance.

    Techniques Used: Immunofluorescence, Imaging, Knockdown, Staining, Anterograde Tracing, Comparison

    Slit3 is specifically knocked out in osteoclasts of Slit3 ‐CKO mice. a) Schematic diagram of Slit3 ‐CKO mice. b) Immunofluorescence of SLIT3 (green) and CD51/61 (red) in subchondral bone. The white dashed line represents the boundary between subchondral bone and cartilage. Scale bars:10 µm. c) Quantitative analysis in panel (b). n = 6. d) qRT‐PCR analysis of the gene expression of Slit3 in subchondral bone in the two groups. n = 3. e,f) Western blot and its quantitative analysis, g) ELISA, and h,i) immunohistochemical staining and quantitative analysis of SLIT3 expression in subchondral bone in the WT and Slit3 ‐CKO groups. n = 3. Scale bars: 70 µm. Statistical analyses were performed using Student's t ‐test. * p < 0.05. ** p < 0.01. *** p < 0.001. **** p <0.0001. ns : no significance.
    Figure Legend Snippet: Slit3 is specifically knocked out in osteoclasts of Slit3 ‐CKO mice. a) Schematic diagram of Slit3 ‐CKO mice. b) Immunofluorescence of SLIT3 (green) and CD51/61 (red) in subchondral bone. The white dashed line represents the boundary between subchondral bone and cartilage. Scale bars:10 µm. c) Quantitative analysis in panel (b). n = 6. d) qRT‐PCR analysis of the gene expression of Slit3 in subchondral bone in the two groups. n = 3. e,f) Western blot and its quantitative analysis, g) ELISA, and h,i) immunohistochemical staining and quantitative analysis of SLIT3 expression in subchondral bone in the WT and Slit3 ‐CKO groups. n = 3. Scale bars: 70 µm. Statistical analyses were performed using Student's t ‐test. * p < 0.05. ** p < 0.01. *** p < 0.001. **** p <0.0001. ns : no significance.

    Techniques Used: Immunofluorescence, Quantitative RT-PCR, Gene Expression, Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining, Expressing



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    Image Search Results


    Pain in TRAP‐Cre; Slit3 −/− mice is reduced. a) Experimental design for TRAP‐Cre transgenic mice injected with AAV9‐Slit3‐RNAi. b) Immunofluorescence of SLIT3 (red) in condyles. The white dashed line represents the boundary between subchondral bone and cartilage. Scale bars:10 µm. c) Quantitative analysis in panel (b). n = 6. d) Representative results of OFT and EPM. e,f) Quantitative analysis of Von Frey experiment (e), OFT and EPM (f). n = 6. Statistical analyses were performed using Student's t ‐test in (c), and one‐way ANOVA with Holm–Šidák multiple comparison tests in (e,f). ** p < 0.01. *** p < 0.001. **** p < 0.0001. ns : no significance.

    Journal: Advanced Science

    Article Title: Osteoclast‐Derived SLIT3 Mediates Osteoarthritis Pain and Degenerative Changes

    doi: 10.1002/advs.202517545

    Figure Lengend Snippet: Pain in TRAP‐Cre; Slit3 −/− mice is reduced. a) Experimental design for TRAP‐Cre transgenic mice injected with AAV9‐Slit3‐RNAi. b) Immunofluorescence of SLIT3 (red) in condyles. The white dashed line represents the boundary between subchondral bone and cartilage. Scale bars:10 µm. c) Quantitative analysis in panel (b). n = 6. d) Representative results of OFT and EPM. e,f) Quantitative analysis of Von Frey experiment (e), OFT and EPM (f). n = 6. Statistical analyses were performed using Student's t ‐test in (c), and one‐way ANOVA with Holm–Šidák multiple comparison tests in (e,f). ** p < 0.01. *** p < 0.001. **** p < 0.0001. ns : no significance.

    Article Snippet: To evaluate the axonal morphology of the TGs in the different groups (SLIT3 protein, RPD353Mu01, Cloud‐clone, USA; Neutralizing antibodies against SLIT3, AF3629, R&D Systems, USA), immunofluorescence labeling (β3‐tubulin, 1:300, 2144, Cell Signaling Technology) and crystal violet staining ( n = 6) were conducted.

    Techniques: Transgenic Assay, Injection, Immunofluorescence, Comparison

    Immunofluorescence imaging and analysis of sensory nerve distribution in the condyle of Slit3 ‐knockdown mice. a) Representative images of immunofluorescence staining for PGP9.5 (red), CGRP (green) and DAPI (blue) along the TMJ osteochondral junction. Scale bars: 10 µm. b) Quantitative analysis of the density of PGP9.5 + nerve fibers and CGRP + nerve fibers in the TMJ osteochondral junction. n = 6. c) Schematic diagram of anterograde tracing. d) Representative images of the condylar sensory nerve. EGFP (rAAV‐CAG‐EGFP‐WPRE‐hGH pA, green), DAPI (blue). Scale bars: 10 µm. e) Quantitative analysis of the density of sensory nerve fibers. n = 6. The white dashed line represents the boundary between bone and cartilage. Statistical analyses were performed using one‐way ANOVA with Holm–Šidák multiple comparison tests. **** p < 0.0001. ns : no significance.

    Journal: Advanced Science

    Article Title: Osteoclast‐Derived SLIT3 Mediates Osteoarthritis Pain and Degenerative Changes

    doi: 10.1002/advs.202517545

    Figure Lengend Snippet: Immunofluorescence imaging and analysis of sensory nerve distribution in the condyle of Slit3 ‐knockdown mice. a) Representative images of immunofluorescence staining for PGP9.5 (red), CGRP (green) and DAPI (blue) along the TMJ osteochondral junction. Scale bars: 10 µm. b) Quantitative analysis of the density of PGP9.5 + nerve fibers and CGRP + nerve fibers in the TMJ osteochondral junction. n = 6. c) Schematic diagram of anterograde tracing. d) Representative images of the condylar sensory nerve. EGFP (rAAV‐CAG‐EGFP‐WPRE‐hGH pA, green), DAPI (blue). Scale bars: 10 µm. e) Quantitative analysis of the density of sensory nerve fibers. n = 6. The white dashed line represents the boundary between bone and cartilage. Statistical analyses were performed using one‐way ANOVA with Holm–Šidák multiple comparison tests. **** p < 0.0001. ns : no significance.

    Article Snippet: To evaluate the axonal morphology of the TGs in the different groups (SLIT3 protein, RPD353Mu01, Cloud‐clone, USA; Neutralizing antibodies against SLIT3, AF3629, R&D Systems, USA), immunofluorescence labeling (β3‐tubulin, 1:300, 2144, Cell Signaling Technology) and crystal violet staining ( n = 6) were conducted.

    Techniques: Immunofluorescence, Imaging, Knockdown, Staining, Anterograde Tracing, Comparison

    Slit3 is specifically knocked out in osteoclasts of Slit3 ‐CKO mice. a) Schematic diagram of Slit3 ‐CKO mice. b) Immunofluorescence of SLIT3 (green) and CD51/61 (red) in subchondral bone. The white dashed line represents the boundary between subchondral bone and cartilage. Scale bars:10 µm. c) Quantitative analysis in panel (b). n = 6. d) qRT‐PCR analysis of the gene expression of Slit3 in subchondral bone in the two groups. n = 3. e,f) Western blot and its quantitative analysis, g) ELISA, and h,i) immunohistochemical staining and quantitative analysis of SLIT3 expression in subchondral bone in the WT and Slit3 ‐CKO groups. n = 3. Scale bars: 70 µm. Statistical analyses were performed using Student's t ‐test. * p < 0.05. ** p < 0.01. *** p < 0.001. **** p <0.0001. ns : no significance.

    Journal: Advanced Science

    Article Title: Osteoclast‐Derived SLIT3 Mediates Osteoarthritis Pain and Degenerative Changes

    doi: 10.1002/advs.202517545

    Figure Lengend Snippet: Slit3 is specifically knocked out in osteoclasts of Slit3 ‐CKO mice. a) Schematic diagram of Slit3 ‐CKO mice. b) Immunofluorescence of SLIT3 (green) and CD51/61 (red) in subchondral bone. The white dashed line represents the boundary between subchondral bone and cartilage. Scale bars:10 µm. c) Quantitative analysis in panel (b). n = 6. d) qRT‐PCR analysis of the gene expression of Slit3 in subchondral bone in the two groups. n = 3. e,f) Western blot and its quantitative analysis, g) ELISA, and h,i) immunohistochemical staining and quantitative analysis of SLIT3 expression in subchondral bone in the WT and Slit3 ‐CKO groups. n = 3. Scale bars: 70 µm. Statistical analyses were performed using Student's t ‐test. * p < 0.05. ** p < 0.01. *** p < 0.001. **** p <0.0001. ns : no significance.

    Article Snippet: To evaluate the axonal morphology of the TGs in the different groups (SLIT3 protein, RPD353Mu01, Cloud‐clone, USA; Neutralizing antibodies against SLIT3, AF3629, R&D Systems, USA), immunofluorescence labeling (β3‐tubulin, 1:300, 2144, Cell Signaling Technology) and crystal violet staining ( n = 6) were conducted.

    Techniques: Immunofluorescence, Quantitative RT-PCR, Gene Expression, Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining, Expressing